Difference between revisions of "Part:BBa K1875003:Experience"
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===Applications of BBa_K1875003=== | ===Applications of BBa_K1875003=== | ||
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| + | ==Characterization by ''' [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] ''' == | ||
| + | We cultured <i>B. xylophilus</i> with <i>S. cerevisiae</i> expressing EGFP of this part from the TDH3 promoter (''' [https://parts.igem.org/Part:BBa_K530008 BBa_K530008] ''' ). After several days we collected the nematodes and observed them with a fluorescence microscope (fig 1 ), and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract. | ||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/8/82/B._xEGFP.jpg" alt="<i>B. xylophilus</i> filled with EGFP in its intestine" style="float:center;width:350px;height:250px;"> | ||
| + | </figure> | ||
| + | Figure1: <i>B. xylophilus</i> full of EGFP in its intestine. | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 02:28, 2 November 2017
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1875003
Characterization by [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017]
We cultured B. xylophilus with S. cerevisiae expressing EGFP of this part from the TDH3 promoter ( BBa_K530008 ). After several days we collected the nematodes and observed them with a fluorescence microscope (fig 1 ), and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract.
User Reviews
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