Difference between revisions of "Part:BBa K2381007"

 
 
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<partinfo>BBa_K2381007 short</partinfo>
 
<partinfo>BBa_K2381007 short</partinfo>
  
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the split dCas9 is expressed alone.
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It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9 and it is cut at amino acid site 56 and 714. The dCas9 is mutated from <i>Streptococcus pyogenes</i>'s nuclease Cas9.
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<h3>References</h3>
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Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.
  
 
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Latest revision as of 02:24, 2 November 2017


Rational split dCas9-2

It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9 and it is cut at amino acid site 56 and 714. The dCas9 is mutated from Streptococcus pyogenes's nuclease Cas9.

References

Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 934
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]