Difference between revisions of "Part:BBa K2210001"

 
 
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<partinfo>BBa_K2210001 short</partinfo>
 
<partinfo>BBa_K2210001 short</partinfo>
  
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An ABC-transporter from L. Lactis
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An ABC-transporter from L. Lactis.
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Please note that this sequence, before modification, is a multidrug resistance factor. When working with this part, RDFZ-China ensured that all bacteria transformed with this part were autoclaved when no longer needed as a safety measure..
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<img src="https://static.igem.org/mediawiki/2017/d/d5/LmrA-BsubtilisPlateRDFZ-China-ControlGroup.jpeg"/>
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<img src="https://static.igem.org/mediawiki/2017/7/79/LmrA-BsubtilisPlateRDFZ-China.jpeg"/>
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The former picture shows the control group, of 5ul culture dripped onto an LB plate. The latter is the picture of B. subtilis transformed with lmrA on the vector pHT01 (promoter: pGrac) dripped onto the plate under the same condition. Both plates were incubated for 24 hours. There are visible differences between the two colonies. The colony in the latter picture seems to have swarming properties, indicating that lmrA may indeed be transporting extra surfactin into the surrounding.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:48, 2 November 2017


lmrA-transporter

An ABC-transporter from L. Lactis.

Please note that this sequence, before modification, is a multidrug resistance factor. When working with this part, RDFZ-China ensured that all bacteria transformed with this part were autoclaved when no longer needed as a safety measure..

<img src="LmrA-BsubtilisPlateRDFZ-China-ControlGroup.jpeg"/> <img src="LmrA-BsubtilisPlateRDFZ-China.jpeg"/>

The former picture shows the control group, of 5ul culture dripped onto an LB plate. The latter is the picture of B. subtilis transformed with lmrA on the vector pHT01 (promoter: pGrac) dripped onto the plate under the same condition. Both plates were incubated for 24 hours. There are visible differences between the two colonies. The colony in the latter picture seems to have swarming properties, indicating that lmrA may indeed be transporting extra surfactin into the surrounding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 697
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
  • 1000
    COMPATIBLE WITH RFC[1000]