Difference between revisions of "Part:BBa K2457005:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | <p><b>Cas9</p></b> | |
− | + | The sequence was ordered as two gBlocks from Integrated DNA Technologies and it was assemble by PvuII restriction enzyme site. | |
− | + | • NheI (GCTAGC), which in the alanine GCT codon (18% de codon usage) was replaced by GCG (32%) | |
+ | • EcoRI (C-GAATTC), which in arginine CGA codon (30%) was replaced by CGT (24.7%) | ||
+ | • First HindIII (AAGCTT) changed to AAACTT | ||
+ | • Second HindIII (AAGCTT) changed to AGGCTT | ||
+ | • NdeI: CATATG changed to CACATG; CATATG changed to CATACG | ||
+ | • NcoI: CCATGG changed to CCGTGG | ||
+ | • BamHI: GGATCC changed to GGACCC | ||
===Source=== | ===Source=== |
Revision as of 01:31, 2 November 2017
Standardized pBAD_Cas9 device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1207
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1042
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1024
Design Notes
Cas9</p> The sequence was ordered as two gBlocks from Integrated DNA Technologies and it was assemble by PvuII restriction enzyme site. • NheI (GCTAGC), which in the alanine GCT codon (18% de codon usage) was replaced by GCG (32%) • EcoRI (C-GAATTC), which in arginine CGA codon (30%) was replaced by CGT (24.7%) • First HindIII (AAGCTT) changed to AAACTT • Second HindIII (AAGCTT) changed to AGGCTT • NdeI: CATATG changed to CACATG; CATATG changed to CATACG • NcoI: CCATGG changed to CCGTGG • BamHI: GGATCC changed to GGACCC
Source
descrever
===References===