Difference between revisions of "Part:BBa K2378009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p>EndA endonuclease is expressed entirely extracellularly. Its natural function in nature is to prevent transformation when expressed, so that <i>Vibrio cholera</i> can manage the period and seasons for transformation.</p> | <p>EndA endonuclease is expressed entirely extracellularly. Its natural function in nature is to prevent transformation when expressed, so that <i>Vibrio cholera</i> can manage the period and seasons for transformation.</p> | ||
Latest revision as of 01:22, 2 November 2017
EndA Endonuclease Coding Device from Vibrio cholera
This is coding device of EndA DNA-specific endonuclease, with constitutive promoter BBa_J61051. The gene is isolated from Vibrio cholera.
Usage and Biology
EndA endonuclease is expressed entirely extracellularly. Its natural function in nature is to prevent transformation when expressed, so that Vibrio cholera can manage the period and seasons for transformation.
Utilizing that characteristics, this enzyme can be used to coat bacterial cells in endonuclease cloud. This can prevent natural transformation from happening. This function is particularly useful as a containment/safety device to prevent transformation of natural organisms by genetically modified ones which can disrupt the natural gene pool.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 305
Illegal NgoMIV site found at 545 - 1000COMPATIBLE WITH RFC[1000]