Difference between revisions of "Part:BBa K2486019"
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<partinfo>BBa_K2486019 short</partinfo> | <partinfo>BBa_K2486019 short</partinfo> | ||
| − | A lactate biosensor composed by parts [https://parts.igem.org/Part: | + | |
| + | A lactate biosensor composed by parts [https://parts.igem.org/Part:BBa_K2486007 BBa_K2486007] and [https://parts.igem.org/Part:BBa_K2486010 BBa_K24860010]. | ||
| + | The part [https://parts.igem.org/Part:BBa_K2486007 BBa_K2486007] works as a LldR generator, one of the repressor proteins capable of blocking gene expression of our [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2486011 PlldR_tetR promoter] (the other protein is TetR). The other componet of our biosensor is the part [https://parts.igem.org/Part:BBa_K2486010 BBa_K24860010] a reporter module composed of our hybrid promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2486011 PlldR_tetR promoter]) and a GFP::lva. | ||
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Revision as of 01:19, 2 November 2017
PlldR_tetR/LldR based lactate biosensor
A lactate biosensor composed by parts BBa_K2486007 and BBa_K24860010. The part BBa_K2486007 works as a LldR generator, one of the repressor proteins capable of blocking gene expression of our PlldR_tetR promoter (the other protein is TetR). The other componet of our biosensor is the part BBa_K24860010 a reporter module composed of our hybrid promoter (PlldR_tetR promoter) and a GFP::lva.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 874
Illegal NheI site found at 897
Illegal NheI site found at 1033 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 284
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1743