Difference between revisions of "Part:BBa K2475002"
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This composite part consists of the Sup35 aggregation domain fused with yellow fluorescent protein (YFP), which is a fluorescent tag with a theoretical excitation peak at 514 nm and emission peak at 535 nm. This was used, in conjunction with PrD-CFP, in Förster resonance energy transfer (FRET) experiments test if/how the aggregation domain affects the energy transfer from CFP to YFP expression in aggregate state (PSI+) and soluble state (PSI-). | This composite part consists of the Sup35 aggregation domain fused with yellow fluorescent protein (YFP), which is a fluorescent tag with a theoretical excitation peak at 514 nm and emission peak at 535 nm. This was used, in conjunction with PrD-CFP, in Förster resonance energy transfer (FRET) experiments test if/how the aggregation domain affects the energy transfer from CFP to YFP expression in aggregate state (PSI+) and soluble state (PSI-). | ||
| + | Sup35 aggregation domain is from <partinfo>BBa_K2475000</partinfo>. | ||
| + | Used with <partinfo>BBa_K2475001</partinfo> to collect FRET data. | ||
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Latest revision as of 01:17, 2 November 2017
Sup 35 Prion Domain fused to Yellow Fluorescent Protein (PrD-YFP)
This composite part consists of the Sup35 aggregation domain fused with yellow fluorescent protein (YFP), which is a fluorescent tag with a theoretical excitation peak at 514 nm and emission peak at 535 nm. This was used, in conjunction with PrD-CFP, in Förster resonance energy transfer (FRET) experiments test if/how the aggregation domain affects the energy transfer from CFP to YFP expression in aggregate state (PSI+) and soluble state (PSI-).
Sup35 aggregation domain is from BBa_K2475000.
Used with BBa_K2475001 to collect FRET data.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 709
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]