Difference between revisions of "Part:BBa K2475001"
 
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<partinfo>BBa_K2475001 short</partinfo>
 
<partinfo>BBa_K2475001 short</partinfo>
  
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This composite part consists of the Sup35 aggregation domain fused with yellow fluorescent protein (YFP), which is a fluorescent tag with a theoretical excitation peak at 514 nm and emission peak at 535 nm. This was used, in conjunction with PrD-CFP, in Förster resonance energy transfer (FRET) experiments test if/how the aggregation domain affects the energy transfer from CFP to YFP expression in aggregate state  (PSI+) and soluble state (PSI-).  
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This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET) experiments to  test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state).
  
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Sup35 aggregation domain is from <partinfo>BBa_K2475000</partinfo>.
  
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Used with <partinfo>BBa_K2475002</partinfo> to collect FRET data.
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2475000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2475001 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 01:17, 2 November 2017


Sup 35 Prion Domain fused to Cyan Fluorescent Protein (PrD-CFP)

This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET) experiments to test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state).

Sup35 aggregation domain is from BBa_K2475000.

Used with BBa_K2475002 to collect FRET data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]