Difference between revisions of "Part:BBa K2455005"
 
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<partinfo>BBa_K2455005 short</partinfo>
 
<partinfo>BBa_K2455005 short</partinfo>
  
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This is an improved version of the mTag BFP ([[Part:BBa_K592100|BBa_K592100]]) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag.  
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This is an improved version of the Blue Fluorescent Protein (mTag BFP; [[Part:BBa_K592100|BBa_K592100]]) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving its associated protein the ability to pass through plasma membranes.
  
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<b>DISCLAIMER:</b> This biobrick carries and illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region.
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For our study we demonstrated that this improved version of [[Part:BBa_K592100|mTag BFP]] had fluorescence of similar levels to the original protein, and was able to stain both <i>Escherichia coli</i> and <i>Pseudomonas putida</i> cells, presumably through internalisation of the protein.
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Previous studies have shown R9 associated proteins to be able to enter a variety of cell types ([https://academic.oup.com/pcp/article/46/3/482/1817347 Chang <i>et al., 2005</i>]).
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<b>DISCLAIMER:</b> This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see [[Part:BBa_K2455002|CPP-SYFP2]].
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K2455005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2455005 SequenceAndFeatures</partinfo>
  

Latest revision as of 01:09, 2 November 2017


CPP-mTag BFP

This is an improved version of the Blue Fluorescent Protein (mTag BFP; BBa_K592100) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving its associated protein the ability to pass through plasma membranes.

For our study we demonstrated that this improved version of mTag BFP had fluorescence of similar levels to the original protein, and was able to stain both Escherichia coli and Pseudomonas putida cells, presumably through internalisation of the protein.

Previous studies have shown R9 associated proteins to be able to enter a variety of cell types (Chang et al., 2005).

DISCLAIMER: This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see CPP-SYFP2.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 741
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 741
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]