Difference between revisions of "Part:BBa K2486007"

 
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<partinfo>BBa_K2486007 short</partinfo>
 
<partinfo>BBa_K2486007 short</partinfo>
  
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This part consists of:
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A part that constitutively generates the LldR repressor
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- A weak promoter from Anderson's Promoter Library (BBa_J23114): You don't want a lot of repressor protein when you design a biosensor, this would make the biosensor less sensitive, so this promoter is adequate for use in biosensor circuits.
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- An RBS based on Elowitz repressilator (BBa_B0034).
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- The regulatory protein LldR (BBa_K1847001) which represses ldhA and lldD, genes that encode for lactate dehydrogenates.
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- A terminator
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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This part consists of:
 +
# A weak promoter from Anderson's Promoter Library (BBa_J23114): You don't want a lot of repressor protein when you design a biosensor, this would make the biosensor less sensitive, so this promoter is adequate for use in biosensor circuits.
 +
# An RBS based on Elowitz repressilator (BBa_B0034).
 +
# The regulatory protein LldR (BBa_K1847001) which represses ldhA and lldD, genes that encode for lactate dehydrogenates.
 +
# A terminator
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2486007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2486007 SequenceAndFeatures</partinfo>

Latest revision as of 00:58, 2 November 2017


lldR repressor generator

A part that constitutively generates the LldR repressor

Usage and Biology

This part consists of:

  1. A weak promoter from Anderson's Promoter Library (BBa_J23114): You don't want a lot of repressor protein when you design a biosensor, this would make the biosensor less sensitive, so this promoter is adequate for use in biosensor circuits.
  2. An RBS based on Elowitz repressilator (BBa_B0034).
  3. The regulatory protein LldR (BBa_K1847001) which represses ldhA and lldD, genes that encode for lactate dehydrogenates.
  4. A terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 874
    Illegal NheI site found at 897
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]