Difference between revisions of "Part:BBa K2365040"
 
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Phosphoglycerate kinase, phosphoglycerate kinase promoter, transcription start key enzyme of glycolysis, and each species to enzyme must survive, the deficiency of the enzyme can cause organism metabolism disorder, as a strong promoter in gene engineering
 
Phosphoglycerate kinase, phosphoglycerate kinase promoter, transcription start key enzyme of glycolysis, and each species to enzyme must survive, the deficiency of the enzyme can cause organism metabolism disorder, as a strong promoter in gene engineering
  
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Artificial promoter from mature yeast constitutively expressed plasmid amplified promoter, even on the expression vector prs423 after insertion of test elements GFP - adh1t fusion fragment into yeast sey6210 test 475-530nm fluorescence intensity.we test TDH3 PGK1 2HXT7
 
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[[File:Ap.png|500px|center]]
 
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File:Ap.png
 
  
  
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<!-- Uncomment this to enable Functional Parameter display
 
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>File:图片1.jpg|500px|center parameters</partinfo>
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<partinfo>BBa_K2365031 parameters</partinfo>
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[[File:启动子全部.jpeg|400px|center]]
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Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. 
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Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

Latest revision as of 00:53, 2 November 2017


PGK1 promoter

PGK1 Yeast promoter Constitutive expression

Phosphoglycerate kinase, phosphoglycerate kinase promoter, transcription start key enzyme of glycolysis, and each species to enzyme must survive, the deficiency of the enzyme can cause organism metabolism disorder, as a strong promoter in gene engineering


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

启动子全部.jpeg

Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.