Difference between revisions of "Part:BBa K2365051"

Latest revision as of 00:50, 2 November 2017

TPI1 promotor

TPI1 Yeast promoter;Triose-phosphateisomerase, triosephosphate isomerase promoter. Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence .It plays an important role in glycolysis, is essential for the energy generation, which has been found in almost all organisms, including mammals, insects, fungi, plants and most bacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 47
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 119

Nature promoter from Saccharomyces cerevisiae genome amplification obtained constitutive promoter, the original gene are associated with the metabolism of yeast.And PCR amplification the cyc1 terminator from the genome,next can form the promoter-cyc1t fusion fragments by overlap extension. Between the elements of the paired homologous arm are reserved for standard cleavage sites, constructed on the expression vector prs423 and inserted GFP (S65T)in the fusion fragments , the recombinant plasmid tansform into yeast sey6210 and test 440-550nm fluorescence intensity.

Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

@@ Line 20: / Line 20: @@
 [[File:TPI1 TPI1-CYC1 NAU-05.jpeg|350px|center]]
-Nature promoter from Saccharomyces cerevisiae genome amplification obtained constitutive promoter, the original gene are associated with the metabolism of yeast. The other life from the genome of Cyc1 was amplified by overlap extension terminator promoter-cyc1t fusion fragments between elements of the same source on the arm pairing reserved standard restriction sites, even on the expression vector prs423 GFP (S65T), inserted into yeast sey6210 test 440-550nm fluorescence intensity.
+Nature promoter from Saccharomyces cerevisiae genome amplification obtained constitutive promoter, the original gene are associated with the metabolism of yeast.And PCR amplification the cyc1 terminator from the genome,next can form the promoter-cyc1t fusion fragments by overlap extension. Between the elements of the paired homologous arm are reserved for standard cleavage sites, constructed on the expression vector prs423 and inserted GFP (S65T)in the fusion fragments , the recombinant plasmid tansform into yeast sey6210 and test 440-550nm fluorescence intensity.
+[[File:启动子全部.jpeg|400px|center]]
-[[File:Nature.png|350px|center]]
+Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h.
+Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.