Difference between revisions of "Part:BBa I737020"

Revision as of 17:25, 26 October 2007

PoPS->Agarase (Digests agar)

This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1861
Illegal PstI site found at 284
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1861
Illegal NheI site found at 106
Illegal NheI site found at 901
Illegal PstI site found at 284
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1861
Illegal XhoI site found at 1964
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1861
Illegal PstI site found at 284
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1861
Illegal PstI site found at 284
Illegal AgeI site found at 368
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1319
Illegal SapI.rc site found at 1717
Illegal SapI.rc site found at 1795

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 __NOTOC__
 <partinfo>BBa_I737020 short</partinfo>
-This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. Currently the gene has a EcorRI site which limits how it can be assembled.
+This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled.
 <!-- Add more about the biology of this part here