Difference between revisions of "Part:BBa K2365033"

 
 
(7 intermediate revisions by 4 users not shown)
Line 5: Line 5:
 
PDC1 Yeast promoter;
 
PDC1 Yeast promoter;
 
Constitutive expression;
 
Constitutive expression;
−
come from Saccharomyces cerevisiae BY4742 genomic sequence
+
come from Saccharomyces cerevisiae BY4742 genomic sequence;
 +
 
 +
pyruvate decarboxylase promoter, can be applied to the formation of CO2 pyruvate and acetaldehyde, relating to a stage of alcohol fermentation, exists in yeast and plants. Widely in a-ketoacid and aldehyde.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 14: Line 16:
 
<partinfo>BBa_K2365033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2365033 SequenceAndFeatures</partinfo>
  
 +
 +
<!-- Uncomment this to enable Functional Parameter display
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2365033 parameters</partinfo>
+
<partinfo>BBa_K2365036 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
PCR result
 +
[[File:Pdc1 pdc1-cyc1.jpeg|300px|center]]
 +
[[File:启动子全部.jpeg|400px|center]]
 +
Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. 
 +
Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

Latest revision as of 00:49, 2 November 2017


PDC1 promotor

PDC1 Yeast promoter; Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence;

pyruvate decarboxylase promoter, can be applied to the formation of CO2 pyruvate and acetaldehyde, relating to a stage of alcohol fermentation, exists in yeast and plants. Widely in a-ketoacid and aldehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 289


PCR result

Pdc1 pdc1-cyc1.jpeg
启动子全部.jpeg

Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.