Difference between revisions of "Part:BBa K2355039"

 
 
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<partinfo>BBa_K2355039 short</partinfo>
 
<partinfo>BBa_K2355039 short</partinfo>
  
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This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is IDIRGPRL.  
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This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>.  
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The specific peptide sequence of the linker is IDIRGPRL.  
  
 
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<partinfo>BBa_K2355039 parameters</partinfo>
 
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<h3>Results of experiments</h3>
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This peptide is not cleaved by any of the three venoms, and can be consequently used as a negative control.
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In the graf below you are able to see the cleavage by all three venoms.
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<html>
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<figure>
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              <img src="https://static.igem.org/mediawiki/2017/f/fd/T--DTU-Denmark--peptidescreenG2.png" alt="Substrate screening data for G2" width="400">
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              <figcaption>Figure 9: Substrate with sequence IDIRGPRL (well G2), incubated with the three different venoms.</figcaption>
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          </figure>
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Latest revision as of 00:23, 2 November 2017


Peptide sequence used for negative control.

This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is IDIRGPRL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

This peptide is not cleaved by any of the three venoms, and can be consequently used as a negative control.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for G2
Figure 9: Substrate with sequence IDIRGPRL (well G2), incubated with the three different venoms.