Difference between revisions of "Part:BBa K2355025"
 
(One intermediate revision by one other user not shown)
Line 21: Line 21:
 
This peptide is cleaved by all three venoms.  
 
This peptide is cleaved by all three venoms.  
 
It can be used as a positive control, or as the start of a decision tree classification.
 
It can be used as a positive control, or as the start of a decision tree classification.
 +
 +
In the graf below you are able to see the cleavage by all three venoms.
 +
<html>
 +
<figure>
 +
              <img src="https://static.igem.org/mediawiki/2017/6/61/T--DTU-Denmark--peptidescreenJ12.png" alt="Substrate screening data for J12" width="400">
 +
                          <figcaption>Figure 1: Substrate with sequence DEMDEKSE (well J12) incubated with the three different venoms.</figcaption>
 +
          </figure>
 +
</html>

Latest revision as of 00:19, 2 November 2017


Cleavable peptide sequence from Human STE20/SPS1 related proline-alanine-rich protein kinase

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is DEMDEKSE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

This peptide is cleaved by all three venoms. It can be used as a positive control, or as the start of a decision tree classification.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for J12
Figure 1: Substrate with sequence DEMDEKSE (well J12) incubated with the three different venoms.