Difference between revisions of "Part:BBa K2355015"

 
 
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<partinfo>BBa_K2355015 short</partinfo>
 
<partinfo>BBa_K2355015 short</partinfo>
  
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This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is LDEDEEDL.
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This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>. The specific peptide sequence of the linker is LDEDEEDL.
  
 
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<partinfo>BBa_K2355015 parameters</partinfo>
 
<partinfo>BBa_K2355015 parameters</partinfo>
 
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<h3>Results of experiments</h3>
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This peptide is cleaved by all three venoms.
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It can be used as a positive control, or as the start of a decision tree classification.
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In the graf below you are able to see the cleavage by all three venoms.
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<html>
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<figure>
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                    <img src="https://static.igem.org/mediawiki/2017/9/9f/T--DTU-Denmark--peptidescreenD20.png" alt="Substrate screening data for D20" width="400">
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              <figcaption>Figure 1: Substrate with sequence LDEDEEDL (well D20) incubated with the three different venoms.</figcaption>
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          </figure>
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</html>

Latest revision as of 00:18, 2 November 2017


Cleavable peptide sequence from Human Ataxin-3

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is LDEDEEDL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

This peptide is cleaved by all three venoms. It can be used as a positive control, or as the start of a decision tree classification.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for D20
Figure 1: Substrate with sequence LDEDEEDL (well D20) incubated with the three different venoms.