Difference between revisions of "Part:BBa K2355008"
 
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<partinfo>BBa_K2355008 short</partinfo>
 
<partinfo>BBa_K2355008 short</partinfo>
  
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This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of <i>Bitis arietans </i>. It is originally from Human Pro-neuropeptide Y, a pro-peptide that is implicated in the secretion of gonadotrophin-release hormone (GnRH)  
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This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of <i>Bitis arietans</i>. It is originally from Human Pro-neuropeptide Y, a pro-peptide that is implicated in the secretion of gonadotrophin-release hormone (GnRH)  
  
 
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<h3>Results of experiments</h3>
 
<h3>Results of experiments</h3>
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This peptide is one out of three peptides that were specifically cleaved only by <i>Bitis arietans</i>.
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In the graf below you are able to see the cleavage by all three venoms.
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<html>
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<figure>
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              <img src="https://static.igem.org/mediawiki/2017/f/f5/T--DTU-Denmark--peptidescreenB17.png" alt="Substrate screening data for B17" width="500">
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              <figcaption>Figure 1: Substrate with sequences RQRYGKRS (well B17) incubated with the three different venoms.</figcaption>
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          </figure>
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</html>

Latest revision as of 00:16, 2 November 2017


Cleavable peptide sequence from Human Pro-neuropeptide Y

This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of Bitis arietans. It is originally from Human Pro-neuropeptide Y, a pro-peptide that is implicated in the secretion of gonadotrophin-release hormone (GnRH)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

This peptide is one out of three peptides that were specifically cleaved only by Bitis arietans.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for B17
Figure 1: Substrate with sequences RQRYGKRS (well B17) incubated with the three different venoms.