Difference between revisions of "Part:BBa K2355026"
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This peptide is one out of three that were specifically cleaved only by <i>Bitis arietans</i>.
 
This peptide is one out of three that were specifically cleaved only by <i>Bitis arietans</i>.
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                            <img src="https://static.igem.org/mediawiki/2017/d/d8/T--DTU-Denmark--peptidescreenK23.png" alt="Substrate screening data for K23" width="500" >
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                            <figcaption>Figure 1: Substrates with sequences PAPDAPLK (well K23) incubated with the three different venoms.</figcaption>
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Revision as of 00:13, 2 November 2017


Cleavable peptide sequence from Human Calcium/calmodulin-dependent protein kinase type IV

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is PAPDAPLK.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Results of experiments

This peptide is one out of three that were specifically cleaved only by Bitis arietans.

Substrate screening data for K23
Figure 1: Substrates with sequences PAPDAPLK (well K23) incubated with the three different venoms.