Difference between revisions of "Part:BBa K2213005"

Revision as of 23:25, 1 November 2017

BBa_K2213005 short ===PduD(1-20)_mCherry_cgPPK===

Not understood

The PduD(1-20) domain is sufficient to localise the part into a recombinant ethanolamine utilisation (Eut) or 1, 2 propanediol (Pdu) bacterial microcompartment (Jakobson et al., 2015). The mCherry allows monitoring the subcellular location of the part. The cgPPK2 domain encodes a soluble class II polyphosphate kinase from Corynebacterium glutamicim(Lindner et al., 2007).

References
Jakobson, C., Kim, E., Slininger, M., Chien, A. and Tullman-Ercek, D. (2015). Localization of Proteins to the 1,2-Propanediol Utilization Microcompartment by Non-native Signal Sequences Is Mediated by a Common Hydrophobic Motif. Journal of Biological Chemistry, 290(40), pp.24519-24533.
Lindner, S., Vidaurre, D., Willbold, S., Schoberth, S. and Wendisch, V. (2007). NCgl2620 Encodes a Class II Polyphosphate Kinase in Corynebacterium glutamicum. Applied and Environmental Microbiology, 73(15), pp.5026-5033.
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 865
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 103
Illegal SapI.rc site found at 1544

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2213005 short
+===PduD(1-20)_mCherry_cgPPK===
 </partinfo>
 The PduD(1-20) domain is sufficient to localise the part into a recombinant ethanolamine utilisation (Eut) or 1, 2 propanediol (Pdu) bacterial microcompartment (Jakobson et al., 2015). The mCherry allows monitoring the subcellular location of the part. The cgPPK2 domain encodes a soluble class II polyphosphate kinase from <i>Corynebacterium glutamicim</i>(Lindner et al., 2007).