Difference between revisions of "Part:BBa K2455003:Design"

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===References===
 
===References===
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Chang, M. <i>et al.</i> (2005). Cellular Internalization of Fluorescent Proteins via Arginine-rich Intracellular Delivery Peptide in Plant Cells. <i>PCP Plant and Cell Physiology</i>, <b>46(3)</b>, pp. 482-8.
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Eudes & Chugh (2008). Cell-penetrating peptides. <i>Plant Signal Behav.</i>, <b>3(8)</b>, pp. 549–550.
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http://www.cbs.dtu.dk/services/AMUSER/
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http://www.cbs.dtu.dk/services/AMUSER/instructions.php

Latest revision as of 23:25, 1 November 2017


Cell-Penetrating USER Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick was created by amplifying a modified version of the pET102 vector with the following set of primers, followed by blunt ligation:

Forward primer: CGTCGCCGTCGCCGT-GAATGCGTGCGATCGCG

Reverse primer: GCGACGGCGACGCAT-GCGTATGTATATCTCCTTCTTAAAG

For creation of a biobrick version the following set of primers was sub-sequently used:

Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC

Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG

Source

The nona-arginine CPP-tags is of synthetic origin, and is inspired by viral transduction domains.

References

Chang, M. et al. (2005). Cellular Internalization of Fluorescent Proteins via Arginine-rich Intracellular Delivery Peptide in Plant Cells. PCP Plant and Cell Physiology, 46(3), pp. 482-8.

Eudes & Chugh (2008). Cell-penetrating peptides. Plant Signal Behav., 3(8), pp. 549–550.

http://www.cbs.dtu.dk/services/AMUSER/

http://www.cbs.dtu.dk/services/AMUSER/instructions.php