Difference between revisions of "Part:BBa K2355002"

 
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<partinfo>BBa_K2355002 parameters</partinfo>
 
<partinfo>BBa_K2355002 parameters</partinfo>
 
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<h3>Results of experiments</h3>
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We aimed to improve the amilCP part from the distribution kit by adding a 6x his-tag to the C-terminal end of the chromoprotein. By doing this, it would theoretically be possible to purify both the amilCP part, and composite parts based on the his-tagged amilCP, using affinity chromatography.
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The first picture is amilCP with His-tag spun down in LB media.
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<p>
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        https://static.igem.org/mediawiki/2017/8/88/T--DTU-Denmark--results-amilcp-assembly-16a.png
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</p>
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The second is a picture of amilCP with His-tag after the lysis procedure by sonication.
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<p>
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        https://static.igem.org/mediawiki/2017/e/ed/T--DTU-Denmark--results-amilcp-assembly-16b.png
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</p>
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It is clear that the expression of the blue/purple color is very strong. In conclusion the amilCP has been successfully modified by adding a His-tag without compromising the protein.

Revision as of 23:24, 1 November 2017


AmilCP with 6x His-tag

This is a modification of the AmilCP part from the Uppsala 2011 team (BBa_K592009). It has a 6x His-tag attached to the C-terminal end of the protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Results of experiments

We aimed to improve the amilCP part from the distribution kit by adding a 6x his-tag to the C-terminal end of the chromoprotein. By doing this, it would theoretically be possible to purify both the amilCP part, and composite parts based on the his-tagged amilCP, using affinity chromatography.

The first picture is amilCP with His-tag spun down in LB media.

T--DTU-Denmark--results-amilcp-assembly-16a.png

The second is a picture of amilCP with His-tag after the lysis procedure by sonication.

T--DTU-Denmark--results-amilcp-assembly-16b.png

It is clear that the expression of the blue/purple color is very strong. In conclusion the amilCP has been successfully modified by adding a His-tag without compromising the protein.