Difference between revisions of "Part:BBa K2448035:Experience"

Revision as of 23:21, 1 November 2017

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Applications of BBa_K2448035

Screening protocol

E. coli cell were transformed either with this part or with it's Dpe mutated version. All the clones present on the plates after the transformation were cultivated and the psicose concentration was evaluated using the protocol described for BBa_K2448025 but in the presence of 50 g/L of fructose per well for 9 to 10 hours as this is the optimal measurement time according to our biosensor characterisation. All tests were performed in technical duplicates. Fluorescence measurements (mCherry) have been normalized on cell density (OD600).

Our Application

Screening of a library of mutants of our enzyme, the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum (BBa_K2448021).

User Reviews

UNIQ668703365952d4c6-partinfo-00000000-QINU UNIQ668703365952d4c6-partinfo-00000001-QINU

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 ===Applications of BBa_K2448035===
+''Screening protocol''
+''E. coli'' cell were transformed either with this part or with it's Dpe mutated version. All the clones present on the plates after the transformation were cultivated and the psicose concentration was evaluated using the protocol described for [[Part:BBa_K2448025|BBa_K2448025]] but in the presence of 50 g/L of fructose per well for 9 to 10 hours as this is the optimal measurement time according to our biosensor characterisation. All tests were performed in technical duplicates. Fluorescence measurements (mCherry) have been normalized on cell density (OD600).
+''Our Application''
+Screening of a library of mutants of our enzyme, the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' ([[Part:BBa_K2448021|BBa_K2448021]]).
 ===User Reviews===