Difference between revisions of "Part:BBa I763010"
(One intermediate revision by one other user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_I763010 short</partinfo> | <partinfo>BBa_I763010 short</partinfo> | ||
− | Promoter lambda - cI regulated - (R0051)regulates the expression of LacI | + | Promoter lambda - cI regulated - (R0051) regulates the expression of LacI repressor protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting plac-driven transcription. |
− | + | ||
Latest revision as of 16:50, 26 October 2007
LacI repressor regulated by Plamda Promoter
Promoter lambda - cI regulated - (R0051) regulates the expression of LacI repressor protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting plac-driven transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1222
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]