Difference between revisions of "Part:BBa K2301001"
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| − | T4 endolysin is a lysozyme isolated from the T4 bacteriophage that lyses a bacteral cell from the inside. It does this by degrading the peptidoglycan layer of the cell wall, exposing the bacteria to the extracellular environment and killing it. The activity of this enzyme can be improved when T4 holin is also present. | + | T4 endolysin is a lysozyme isolated from the T4 bacteriophage that lyses a bacteral cell from the inside. It does this by degrading the peptidoglycan layer of the cell wall, exposing the bacteria to the extracellular environment and killing it. The activity of this enzyme can be improved when T4 holin is also present. We incorporated this part along with BBa_K2301000, BBa_K2301002 and BBa_K2301003 into a temperature controlled kill switch for bacteria. |
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Latest revision as of 23:13, 1 November 2017
T4 Endolysin optimized for expression in E. coli
T4 endolysin is a lysozyme isolated from the T4 bacteriophage that lyses a bacteral cell from the inside. It does this by degrading the peptidoglycan layer of the cell wall, exposing the bacteria to the extracellular environment and killing it. The activity of this enzyme can be improved when T4 holin is also present. We incorporated this part along with BBa_K2301000, BBa_K2301002 and BBa_K2301003 into a temperature controlled kill switch for bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 259
Illegal AgeI site found at 329 - 1000COMPATIBLE WITH RFC[1000]