Difference between revisions of "Part:BBa K2295002"
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− | Hypoxia-inducible factors (HIFs) are transcription factors responding to decreased | + | Hypoxia-inducible factors (HIFs) are transcription factors responding to decreased oxygen levels in the cellular environment. |
− | Hypoxia-inducible factor 1-alpha (HIF1A) is constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. In hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (<partinfo>BBa_K2295003</partinfo>) in the nucleus | + | Hypoxia-inducible factor 1-alpha (HIF1A) is constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. In hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (<partinfo>BBa_K2295003</partinfo>) in the nucleus (Ziello et al., 2007). |
===Western Blot against HIF1A=== | ===Western Blot against HIF1A=== |
Revision as of 23:09, 1 November 2017
HIF1A (Hypoxia-inducible factor one alpha)
Mechanism
Hypoxia-inducible factors (HIFs) are transcription factors responding to decreased oxygen levels in the cellular environment. Hypoxia-inducible factor 1-alpha (HIF1A) is constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. In hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (BBa_K2295003) in the nucleus (Ziello et al., 2007).
Western Blot against HIF1A
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]