Difference between revisions of "Part:BBa K2450251"
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A Tet Repressor tagged with CFP. TetR binds to the tet operator to prevent the transcription of a downstream gene. Repression is relieved by tetracycline.
 
A Tet Repressor tagged with CFP. TetR binds to the tet operator to prevent the transcription of a downstream gene. Repression is relieved by tetracycline.
  
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K2450251 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2450251 SequenceAndFeatures</partinfo>
  
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TetR functions as a homodimer to bind tet operator sequences and prevent the association of RNA polymerase with its promoter. Each TetR monomer has a DNA binding domain and a dimerisation domain. The DNA binding domain is a helix-turn-helix motif that associates with a tet operator sequence (TCCCTATCAGTGATAGAGA). Our complementary part, BBa_K2450301, contains 2 repeats of this sequence - one for each monomer.
 
TetR functions as a homodimer to bind tet operator sequences and prevent the association of RNA polymerase with its promoter. Each TetR monomer has a DNA binding domain and a dimerisation domain. The DNA binding domain is a helix-turn-helix motif that associates with a tet operator sequence (TCCCTATCAGTGATAGAGA). Our complementary part, BBa_K2450301, contains 2 repeats of this sequence - one for each monomer.
  
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CFP is a variation on Green Fluorescent Protein.
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For more information on how this part was used in our project, please see our wiki: http://2017.igem.org/Team:Oxford
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2450251 parameters</partinfo>
 
<partinfo>BBa_K2450251 parameters</partinfo>
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Latest revision as of 22:59, 1 November 2017


TetR CFP

A Tet Repressor tagged with CFP. TetR binds to the tet operator to prevent the transcription of a downstream gene. Repression is relieved by tetracycline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

TetR functions as a homodimer to bind tet operator sequences and prevent the association of RNA polymerase with its promoter. Each TetR monomer has a DNA binding domain and a dimerisation domain. The DNA binding domain is a helix-turn-helix motif that associates with a tet operator sequence (TCCCTATCAGTGATAGAGA). Our complementary part, BBa_K2450301, contains 2 repeats of this sequence - one for each monomer.

CFP is a variation on Green Fluorescent Protein.

For more information on how this part was used in our project, please see our wiki: http://2017.igem.org/Team:Oxford

Functional Parameters