Difference between revisions of "Part:BBa K2442203"
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The intergenic region lies between the mtlR stop codon and the mtlE start codon (158 bp). Within this intergenic region the mtle promoter resides <i>Figure 1</i> illustrates where the promoter is thought to lie in the region relative to the neighboring coding sequences. | The intergenic region lies between the mtlR stop codon and the mtlE start codon (158 bp). Within this intergenic region the mtle promoter resides <i>Figure 1</i> illustrates where the promoter is thought to lie in the region relative to the neighboring coding sequences. | ||
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https://static.igem.org/mediawiki/2017/7/7b/T-Glasgow-intergenicmtle.jpeg. | https://static.igem.org/mediawiki/2017/7/7b/T-Glasgow-intergenicmtle.jpeg. | ||
Revision as of 22:05, 1 November 2017
Pmtle +RBS WT
This part contains the native pmtlE promoter and its native ribosome binding site. pmtlE is the intergenic region between mtlR and the mtlE coding region in Pseudomonas Flourescens. Naturally the pmtle promoter is regulated by the mtlR protein which complexes upon induction by sugars and activates the promoter.
The intergenic region lies between the mtlR stop codon and the mtlE start codon (158 bp). Within this intergenic region the mtle promoter resides Figure 1 illustrates where the promoter is thought to lie in the region relative to the neighboring coding sequences.
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For this part the entire intergenic region (including the pmtle promoter) was cloned into psb1c3. Within this sequence there is a putative wild type ribosome binding site. This part was one of the variants tested with mtlR to evoke a GFP response via sugar induction.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]