Difference between revisions of "Part:BBa K2355317"

 
 
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<partinfo>BBa_K2355317 short</partinfo>
 
<partinfo>BBa_K2355317 short</partinfo>
  
−
Scar for part BBa_K2355302
+
The cut side that allows insertions of RFC[25] parts.
 +
The cut side are NgoMIV and AgeI, between the cut sides there is a stop codon and a frameshift.
 +
If insertion of RFC[25] part is not succesful the succeeding part will not be expressed.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:52, 1 November 2017


Double cut side containing NgoMIV and AgeI, with a frameshift and stop codon in between.

The cut side that allows insertions of RFC[25] parts. The cut side are NgoMIV and AgeI, between the cut sides there is a stop codon and a frameshift. If insertion of RFC[25] part is not succesful the succeeding part will not be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1
    Illegal AgeI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 12