Difference between revisions of "Part:BBa K2205005"
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As an improvement on the Tokyo Tech 2015 team part [https://parts.igem.org/Part:BBa_K1632011 BBa_K1632011]The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to <i>rhlI</i> (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.<br/><br/> | As an improvement on the Tokyo Tech 2015 team part [https://parts.igem.org/Part:BBa_K1632011 BBa_K1632011]The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to <i>rhlI</i> (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.<br/><br/> | ||
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Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205015) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.<br/><br/> | Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205015) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.<br/><br/> | ||
Latest revision as of 21:43, 1 November 2017
Fim Standby Switch
As an improvement on the Tokyo Tech 2015 team part BBa_K1632011The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to rhlI (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.
Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205015) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]