Difference between revisions of "Part:BBa K1491017"

Latest revision as of 21:40, 1 November 2017

Codon Optimized Supernova

Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.

Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).

Improvement

2017 SSTi-SZGD Team has improved the this part. The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff BBa_K2244009. we gave it a new name supernova-lev1(BBa_K2244010)

2017 NU_Kazakhstan Team has improved the function of SuperNova protein for killing the cell by making its membrane-bound version in Chlamydomonas reinhardtii. Link: BBa_K2516003.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 50
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <hr>
 <center>https://static.igem.org/mediawiki/2014/thumb/d/dc/ConstitutiveSuperoxides.jpg/386px-ConstitutiveSuperoxides.jpg</center>
+===Improvement===
+SSTi-SZGD Team has improved the this part. The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff BBa_K2244009. we gave it a new name supernova-lev1([https://parts.igem.org/Part:BBa_K2244010 BBa_K2244010])
+NU_Kazakhstan Team has improved the function of SuperNova protein for killing the cell by making its membrane-bound version in <i>Chlamydomonas reinhardtii</i>. Link: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2516003 BBa_K2516003].
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 <partinfo>BBa_K1491017 parameters</partinfo>
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-===improvement===
-SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009), cell apoptosis can be induced by firstly expressing supernova gene in darkness, and then expose cells to light illumination that results to ROS release and the subsequent cell death. The entire process is purely regulated by light. We believe this has improved the original use of supernova in a T7 system using IPTG induction, which requires additional chemical inducer.