Difference between revisions of "Part:BBa K2384000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Our sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and we add a 6*His tag at the C end to facilitate purification of protein.
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Our sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and there is no termination codon with this DNA in pSB1C3 plasmid.  
  
  
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The original sequence of this gene has been learned from the web page of the Nanjing Agricultural University team in 2014 and sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and we add a 6*His tag at the C end to facilitate purification of protein.
 
The original sequence of this gene has been learned from the web page of the Nanjing Agricultural University team in 2014 and sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and we add a 6*His tag at the C end to facilitate purification of protein.
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This gene is composed of synthetic biology company.
  
 
===References===
 
===References===

Latest revision as of 21:13, 1 November 2017


T7 RNA polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and there is no termination codon with this DNA in pSB1C3 plasmid.


Source

The original sequence of this gene has been learned from the web page of the Nanjing Agricultural University team in 2014 and sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and we add a 6*His tag at the C end to facilitate purification of protein. This gene is composed of synthetic biology company.

References