Difference between revisions of "Part:BBa K2515003:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This part was PCR amplified using BBa_K145201 as matrix and then cloned in pSB1C3.
 
  
 +
<br /><br />
 +
This part was PCR amplified using BBa_K145201 as matrix and then cloned in pSB1C3.
 +
<br /><br />
 +
To clone this part by yourself, use the following primer pair:
 +
<br /><br />
 +
Tet_Gen_InvF TATCTGCAGCGGCCGCTACTAGTAttgacagctagctcagtcc<br />
 +
Tet_Gen_InvR TATGAATTCGCGGCCGCTTCTAGAGTTAcccactttcacatttaagttg<br />
 +
<br />
  
 +
The resulting product should be purified, digested with EcoRI and PstI and ligated in pSB vector of choice.
 +
<br /><br />
 +
<b>Important:</b> In contrast to the original part, this one has TetR with no LVA-like tag and no transcriptional terminators. If you want to add terminator, we do recommend part Part:BBa_B0025.
  
 
===Source===
 
===Source===

Revision as of 21:09, 1 November 2017


Inverted INPUT TetR generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes



This part was PCR amplified using BBa_K145201 as matrix and then cloned in pSB1C3.

To clone this part by yourself, use the following primer pair:

Tet_Gen_InvF TATCTGCAGCGGCCGCTACTAGTAttgacagctagctcagtcc
Tet_Gen_InvR TATGAATTCGCGGCCGCTTCTAGAGTTAcccactttcacatttaagttg

The resulting product should be purified, digested with EcoRI and PstI and ligated in pSB vector of choice.

Important: In contrast to the original part, this one has TetR with no LVA-like tag and no transcriptional terminators. If you want to add terminator, we do recommend part Part:BBa_B0025.

Source

Part:BBa_K145201

References