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Contribution from uOttawa iGEM 2017
 
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<br>Author: Setti Belhouari  
 
<br>Author: Setti Belhouari  
 
<br>Biobrick <partinfo>BBa_B0031</partinfo> has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.  
 
<br>Biobrick <partinfo>BBa_B0031</partinfo> has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.  

Revision as of 20:59, 1 November 2017

mRFP protein generator TERM+ RBS+(B0031)


Red fluorescent protein generator with BBa_B0031 low efficiency RBS (from Community Collection) and double terminator.
This construct is useful to measure the relative strength of promoters or the RBS efficiency in relationship with a promoter of interest.
To build a measurement device is only necessary assemble a standard biobrick promoter with this device (or another one of the collection: BBa_K516030 ; BBa_K516032)

Contribution from uOttawa iGEM 2017 T--uOttawa--neg ctrl o,2f,n 008.png T--uOttawa--Tube 001 001.png T--uOttawa--Tube 002 002.png T--uOttawa--Tube 003 003.png T--uOttawa--Tube 004 004.png T--uOttawa--Tube 005 005.png T--uOttawa--Tube 006 006.png T--uOttawa--Tube 007 009.png T--uOttawa--Tube 008 010.png T--uOttawa--Tube 009 011.png
Author: Setti Belhouari
Biobrick BBa_B0031 has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.
Picture of plate.
CSV files.
Histograms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 575
    Illegal AgeI site found at 687
  • 1000
    COMPATIBLE WITH RFC[1000]