Difference between revisions of "Part:BBa J63005:Experience"
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===NAU-CHINA 2017=== | ===NAU-CHINA 2017=== | ||
| − | [[File: | + | [[File:ADH1 promoter.jpeg|500px|center]] |
| + | Figure.Fluorescence intensity of different colonies with truncated ADH1 promoter | ||
| + | Improved design: | ||
| + | We construct the device： | ||
| + | ADH1 promoter+GFP+CYC1 terminator | ||
| + | ADH1 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence. | ||
| + | We transformed this plasmid into Saccharomyces cerevisiae SEY6210. | ||
| + | The promoter of yeast alcohol dehydrogenase (ADH1) is widely used for the expression of heterologous genes in Saccharomyces cerevisiae. We tested the expression of GFP proteins in five transformants containing the ADH1 promoter. Our data shows that there are some differences in the fluorescence intensity of different yeast transformants with ADH1 promoter. The variation trends of promoter strengths from early-log to stationary phase varied in different transformants. Individual transformants colonies have different expression efficiency. We will test this promoter for more than three times. | ||
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NAU-CHINA 2017
Figure.Fluorescence intensity of different colonies with truncated ADH1 promoter
Improved design: We construct the device： ADH1 promoter+GFP+CYC1 terminator ADH1 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence. We transformed this plasmid into Saccharomyces cerevisiae SEY6210. The promoter of yeast alcohol dehydrogenase (ADH1) is widely used for the expression of heterologous genes in Saccharomyces cerevisiae. We tested the expression of GFP proteins in five transformants containing the ADH1 promoter. Our data shows that there are some differences in the fluorescence intensity of different yeast transformants with ADH1 promoter. The variation trends of promoter strengths from early-log to stationary phase varied in different transformants. Individual transformants colonies have different expression efficiency. We will test this promoter for more than three times.