Difference between revisions of "Part:BBa K2271106:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | |||
− | + | <html><p> | |
− | + | The peroxisomal targeting signal 1 supposedly consists of 12 amino acids(<a href="https://www.ncbi.nlm.nih.gov/pubmed/26359497"><abbr title="Structural Insights into Cargo Recognition by the Yeast PTS1 | |
− | === | + | Receptor">Erdmann et al.,2015</abbr></a>). We did research on the amino acid composition naturally occuring in Pts1 sequences (<a href="https://www.ncbi.nlm.nih.gov/pubmed/9837948"><abbr title="The Difference in Recognition of Terminal Tripeptides as |
− | + | Peroxisomal Targeting Signal 1 between Yeast and Human | |
− | + | Is Due to Different Affinities of Their Receptor Pex5p to the | |
+ | Cognate Signal and to Residues Adjacent to It">Lametschwandter <i>et al.</i>, 1998</abbr></a>) and designed our Pts1 sequences with respect to that, just modifying the last four amino acids. | ||
+ | </p></html> | ||
===References=== | ===References=== | ||
+ | <html><p> | ||
+ | Structural Insights into Cargo Recognition by the Yeast PTS1 | ||
+ | Receptor - Erdmann <i>et al.</i>, 2015 | ||
+ | <br> | ||
+ | The Difference in Recognition of Terminal Tripeptides as | ||
+ | Peroxisomal Targeting Signal 1 between Yeast and Human | ||
+ | Is Due to Different Affinities of Their Receptor Pex5p to the | ||
+ | Cognate Signal and to Residues Adjacent to It - Lametschwandter <i>et al.</i>, 1998 | ||
+ | </p> | ||
+ | </html> |
Latest revision as of 20:51, 1 November 2017
PTS1 variant P3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The peroxisomal targeting signal 1 supposedly consists of 12 amino acids(Erdmann et al.,2015). We did research on the amino acid composition naturally occuring in Pts1 sequences (Lametschwandter et al., 1998) and designed our Pts1 sequences with respect to that, just modifying the last four amino acids.
References
Structural Insights into Cargo Recognition by the Yeast PTS1
Receptor - Erdmann et al., 2015
The Difference in Recognition of Terminal Tripeptides as
Peroxisomal Targeting Signal 1 between Yeast and Human
Is Due to Different Affinities of Their Receptor Pex5p to the
Cognate Signal and to Residues Adjacent to It - Lametschwandter et al., 1998