Difference between revisions of "Part:BBa K2486176:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The design of this part was inspired by the synthetic toehold switch regulated by a T7 promoter. Since we intended to use this part in another chassis, we decided to replace the T7 promoter with a pLac promoter along with a new terminator.  
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The design of this part was inspired by the synthetic toehold switch regulated by a T7 promoter. Since we intended to use this part in another chassis, we decided create this part to be used along with other promoters.
  
  

Latest revision as of 20:21, 1 November 2017


Synthetic Toehold Switch RNA with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


Design Notes

The design of this part was inspired by the synthetic toehold switch regulated by a T7 promoter. Since we intended to use this part in another chassis, we decided create this part to be used along with other promoters.


Source

Adapted from: Green, A., Silver, P., Collins, J., & Yin, P. (n.d.). Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell, 925-939.

References