Difference between revisions of "Part:BBa K2271003"
Pauline tot (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2271003 short</partinfo> | <partinfo>BBa_K2271003 short</partinfo> | ||
| − | - | + | This part contains the coding sequence for the peroxin Pex11p with the galactose inducible promoter ScGAL1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
<!-- --> | <!-- --> | ||
Revision as of 20:18, 1 November 2017
Pex11-Venus with galactose inducible promoter
This part contains the coding sequence for the peroxin Pex11p with the galactose inducible promoter ScGAL1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 534
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]