Difference between revisions of "Part:BBa K1039031"
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<partinfo>BBa_K1039031 short</partinfo> | <partinfo>BBa_K1039031 short</partinfo> | ||
| − | . | + | The attB site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR). |
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| + | =Characterization by Fudan_China 2017= | ||
| + | *'''Group:''' Fudan_China 2017 | ||
| + | *'''Author:''' Haiyun Liu, Fudan University | ||
| + | We characterized the kinetic characterization and the orthogonality between Bxb1 and some other serine recombinase using this attP site. For more information, go to [[Part:BBa_K907000|Mycobacterium Phage Bxb1 gp35, DNA integrase]] | ||
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Latest revision as of 20:18, 1 November 2017
BXB1 att P
The attB site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).
Characterization by Fudan_China 2017
- Group: Fudan_China 2017
- Author: Haiyun Liu, Fudan University
We characterized the kinetic characterization and the orthogonality between Bxb1 and some other serine recombinase using this attP site. For more information, go to Mycobacterium Phage Bxb1 gp35, DNA integrase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 27