Difference between revisions of "Part:BBa K2365512"

Revision as of 20:09, 1 November 2017

MtDef4

MtDef4 is a 47-amino acid protein that is expressed constitutively and in response to biotic and abiotic stress in many tissues of a model legume, M.truncatula.Plant defensin MtDef4 is potent inhibitors of the growth of several filamentous fungi including Fusarium graminearum,and it is a nonmorphogenic defensin that does not induce hyperbranching.But it can induce plasma membrane permeabilization in Fusarium graminearum.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

The result shows the gene amplification result of MtDef4

3-dimension structure and plasmid stucture

A schematic representation of our basic plasmid types, the response construct. It includes the anti-fungal peptide coding gene (anti-fungal peptide) and the inducible promoter. This construct allows genes placed downstream of the inducible promoter to be transcribed when the yeast cells are induced with the appropriate inducer—methanol.

SDS-PAGE

HPLC Analysis Report

MS Analysis Report

Zone of inhibition

The inhibition zone results show the antifungal peptide can exhibit a great antifungal activity, and the area of the inhibition zone is dose dependent. We choose to utilize sterile water as our negative control and 100ppm carbendazim dissolved in acetic acid as positive control. MtDef4 antifungal peptide is dissolved in sterile water.

OD 595nm

We test the cell viability under the action of a series of different concentrations of antifungal peptide. MtDef4 possess great antifungal activity

@@ Line 30: / Line 30: @@
 ====HPLC Analysis Report====
 [[File:HPLC Analysis M.jpeg|550px|center]]
+====MS Analysis Report====
+[[File:MS Report M.jpeg|550px|center]]
 ====Zone of inhibition====
 [[File:MtDef4 inhibition zone.png|500px|center]]