Difference between revisions of "Part:BBa K2267043"
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Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. | Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. | ||
| − | In this case, the constitutively expressed LuxR transcriptional regulator ( | + | In this case, the constitutively expressed LuxR transcriptional regulator (part1: https://parts.igem.org/Part:BBa_K2267029 ) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device. |
| − | + | https://parts.igem.org/Part:BBa_K2267040 is Control， | |
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part1: https://parts.igem.org/Part:BBa_K2267043 | part1: https://parts.igem.org/Part:BBa_K2267043 | ||
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part8: https://parts.igem.org/Part:BBa_K2267045 | part8: https://parts.igem.org/Part:BBa_K2267045 | ||
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===result=== | ===result=== | ||
Latest revision as of 19:34, 1 November 2017
LuxR-I-Plux-GFP-1
This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.
Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. In this case, the constitutively expressed LuxR transcriptional regulator (part1: https://parts.igem.org/Part:BBa_K2267029 ) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.
https://parts.igem.org/Part:BBa_K2267040 is Control，
part1: https://parts.igem.org/Part:BBa_K2267043
part3: https://parts.igem.org/Part:BBa_K2267041
part4: https://parts.igem.org/Part:BBa_K2267042
part6: https://parts.igem.org/Part:BBa_K2267044
part8: https://parts.igem.org/Part:BBa_K2267045
result
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 837
Illegal NheI site found at 860 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 928
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1452
Illegal BsaI.rc site found at 2178