Difference between revisions of "Part:BBa K2205027"

 
 
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<partinfo>BBa_K2205027 short</partinfo>
 
<partinfo>BBa_K2205027 short</partinfo>
  
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Description
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===Description===
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<p>The part [https://parts.igem.org/Part:BBa_K2205024 BBa_K2205024] was designed as Processor unit of the Sensynova multicellular framework for biosensors development (Figure 1). </p>
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[[File:Framework_generic.jpg|400px|]]
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<p>This part is the intermediate part produced in the making of part [https://parts.igem.org/Part:BBa_K2205024 BBa_K2205024] which was made to test the modularity of the system by inserting the P2 Phage Sensitivity Tuner (BBa_ K274371) construct between the two parts of the empty processing module of the Sensynova platform. </p>
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<p>In this part (BBa_K2205027), the part [https://parts.igem.org/Part:BBa_K274371 BBa_ K274371] is under the control of our connector 1, part [https://parts.igem.org/Part:BBa_K2205010 BBa_K2205010], however, not yet controlling our connector 2, part [https://parts.igem.org/Part:BBa_K2205011 BBa_K2205011]. </p>
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<p>When complete, the system will work through the repression of the part BBa_ K274371 except in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform. </p>
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===References===
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iGEM Community. (2009). Team Cambridge 2009. [online] Available at: http://2009.igem.org/Team:Cambridge [Accessed 30 Oct. 2017].
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===Usage and Biology===
 
  
 
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Latest revision as of 19:15, 1 November 2017


Sensynova Framework (P2 ogr signal tuner Processing Module - pCell) - connector 1B ONLY

Description

The part BBa_K2205024 was designed as Processor unit of the Sensynova multicellular framework for biosensors development (Figure 1).

Framework generic.jpg

This part is the intermediate part produced in the making of part BBa_K2205024 which was made to test the modularity of the system by inserting the P2 Phage Sensitivity Tuner (BBa_ K274371) construct between the two parts of the empty processing module of the Sensynova platform.

In this part (BBa_K2205027), the part BBa_ K274371 is under the control of our connector 1, part BBa_K2205010, however, not yet controlling our connector 2, part BBa_K2205011.

When complete, the system will work through the repression of the part BBa_ K274371 except in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform.

References

iGEM Community. (2009). Team Cambridge 2009. [online] Available at: http://2009.igem.org/Team:Cambridge [Accessed 30 Oct. 2017].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]