Difference between revisions of "Part:BBa K2384006"
 
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<b>Optimization Report</b>
 
<b>Optimization Report</b>
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Expression System:Bacillus megaterium
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Gene Length:189 (bp)
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1.Codon Used Adjustment
 
1.Codon Used Adjustment
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<table><tr>
 
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<th>[[Image:T--FAFU-CHINA--cspc1.png|thumb|400px|'''Before Codon Adjustment''']]</th>
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<th>[[Image:T--FAFU-CHINA--cspc1.png|thumb|400px|'''Figure 1:Before Codon Adjustment''']]</th>
 
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<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--cspc2.png|thumb|400px|'''After Codon Adjustment''']]</th>
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<th>[[Image:T--FAFU-CHINA--cspc2.png|thumb|400px|'''Figure 2:After Codon Adjustment''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--cspc3.png|thumb|400px|'''Before Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--cspc3.png|thumb|400px|'''Figure 3:Before Optimization''']]</th>
 
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--cspc4.png|thumb|400px|'''After Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--cspc4.png|thumb|400px|'''Figure 4:After Optimization''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--cspc5.png|thumb|400px|'''Before Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--cspc5.png|thumb|400px|'''Figure 5:Before Optimization''']]</th>
 
</tr></table>
 
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<table><tr>
 
<table><tr>
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<th>[[Image:T--FAFU-CHINA--cspc6.png|thumb|400px|'''After Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--cspc6.png|thumb|400px|'''Figure 6:After Optimization''']]</th>
 
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<th>[[Image:T--FAFU-CHINA--cspc7-1.png|thumb|400px|'''After Optimization''']]</th>
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<th>[[Image:T--FAFU-CHINA--cspc7-1.png|thumb|400px|'''Figure 7:After Optimization''']]</th>
 
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<table><tr>
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<th>[[Image:T--FAFU-CHINA--M-CSPC.jpg|thumb|400px|'''Figure 8: Gene on pSB1C3 Digested with SpeI''']]</th>
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 19:00, 1 November 2017


Pveg+RBS+CspC+6*HIS+T1 terminator+T7 terminator


This is an improve part.This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii.It can largely increase the cold resistance of bacterias.CDS sequence optimization in (BBa_K538004) is better suited for expression in Bacillus megaterium and we add a 6*His tag at the C end.After that, the promoter (BBa_K143012) and RBS (BBa_K090505) were combined.


Optimization Report

Expression System:Bacillus megaterium

Gene Length:189 (bp)


1.Codon Used Adjustment The best value is 1 for sequence optimization.


Figure 1:Before Codon Adjustment
Figure 2:After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Figure 3:Before Optimization
Figure 4:After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Figure 5:Before Optimization
Figure 6:After Optimization

4.Restriction Enzyme

Figure 7:After Optimization
Figure 8: Gene on pSB1C3 Digested with SpeI

Usage and Biology

Freeze/thaw cycle survival rate of Bacillus megaterium containing an empty vector (red line), a vector carrying CspC gene (blue line).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]