Difference between revisions of "Part:BBa K2448034:Design"
 
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Latest revision as of 18:49, 1 November 2017


D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 208
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 619
    Illegal AgeI site found at 646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


Design Notes

RBS (BBa_K2448020) + D-psicose 3-epimerase from Agrobacterium tumefaciens coding sequence (BBa_K2448022)

Source

PCR amplification of the Atu4750 gene from the Agrobacterium tumefaciens str. C58 genome using a forward primer containing the RBS sequence.

References

[1] Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK. Characterization of an Agrobacterium tumefaciens D-psicose 3-epimerase that converts D-fructose to D-psicose. Appl Environ Microbiol. (2006) 72, 981-985.

[2] Kim K, Kim HJ, Oh DK, Cha SS, Rhee S. Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes. J Mol Biol. (2006) 361, 920-931.