Difference between revisions of "Part:BBa K2407306"
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| − | + | ==Introduction== | |
| + | It is regulated by TEF1 promoter. This composite workes for inserting the vox sites and Ura3 gene between them can be replaced by any structural gene. Initially, Before the vika-vox system works, the β-carotene does not express. After mating, the component between two vox sites will be deleted by vika enzyme and the β-carotene gene will synthesize β-carotene. Yeasts turns to orange. | ||
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Latest revision as of 18:41, 1 November 2017
TEF Promoter + vox + Ura3 + Ura3 terminator + vox + β-carotene
Introduction
It is regulated by TEF1 promoter. This composite workes for inserting the vox sites and Ura3 gene between them can be replaced by any structural gene. Initially, Before the vika-vox system works, the β-carotene does not express. After mating, the component between two vox sites will be deleted by vika enzyme and the β-carotene gene will synthesize β-carotene. Yeasts turns to orange.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2145
Illegal BglII site found at 3832
Illegal XhoI site found at 1844
Illegal XhoI site found at 1976
Illegal XhoI site found at 2795 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2012
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1125
Illegal BsaI site found at 4736
Illegal BsaI site found at 7091
Illegal BsaI.rc site found at 176
Illegal BsaI.rc site found at 1572
Illegal BsaI.rc site found at 7422
Illegal SapI site found at 4136
Illegal SapI.rc site found at 972