Difference between revisions of "Part:BBa K2443009"
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<partinfo>BBa_K2443009 short</partinfo> | <partinfo>BBa_K2443009 short</partinfo> | ||
| − | Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. | + | Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of HisRS is 48 kDa, which corresponds to the band seen in lane 3 of the overexpression gel below. Overexpression was performed from BL21DE3 gold cells from a pJET vector according to the overexpression protocol, and notebook on the 2017 iGEM Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf). |
| + | https://static.igem.org/mediawiki/parts/b/bf/Lethbridge_Oct_22_gel.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 18:24, 1 November 2017
HisRS optimized for expression in E. coli
Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of HisRS is 48 kDa, which corresponds to the band seen in lane 3 of the overexpression gel below. Overexpression was performed from BL21DE3 gold cells from a pJET vector according to the overexpression protocol, and notebook on the 2017 iGEM Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]