Difference between revisions of "Part:BBa K2443009"

Latest revision as of 18:24, 1 November 2017

HisRS optimized for expression in E. coli

Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of HisRS is 48 kDa, which corresponds to the band seen in lane 3 of the overexpression gel below. Overexpression was performed from BL21DE3 gold cells from a pJET vector according to the overexpression protocol, and notebook on the 2017 iGEM Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2443009 short</partinfo>
-Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
+Histidine tRNA synthetase responsible for attaching histidine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of HisRS is 48 kDa, which corresponds to the band seen in lane 3 of the overexpression gel below. Overexpression was performed from BL21DE3 gold cells from a pJET vector according to the overexpression protocol, and notebook on the 2017 iGEM Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
+https://static.igem.org/mediawiki/parts/b/bf/Lethbridge_Oct_22_gel.png
 <!-- Add more about the biology of this part here