Difference between revisions of "Part:BBa K2213004"
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| − | <img src= "https://static.igem.org/mediawiki/2017/ | + | <img src= "https://static.igem.org/mediawiki/2017/a/a8/PduD%281-20%29_cgPPK_mCherry_%283%29.png" width="362" height="404"> |
| − | <p><font color="#111"><center></h3><b>Figure 1.</b> SDS-PAGE analysis of fractions from the Ni-NTA agarose purification of the | + | <p><font color="#111"><center></h3><b>Figure 1.</b> SDS-PAGE analysis of fractions from the Ni-NTA agarose purification of the PduD(1-20)_cgPPK_mCherry construct. The black arrow indicates where the expected band should be (66 kDa) in the elution fraction lane. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 18:23, 1 November 2017
PduD(1-20)_cgPPK2_mCherry_His6
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Usage and Biology
Fusion of the PduD(1-20), Corynebacterium glutamicum class II polyphosphate kinase and mCherry followed by a hexahistidine tag.
The construct was heterologously expressed in a BL21 (DE3) strain of E. coli under the control of a T7 promoter. The construct was cleaved before or during purification, resulting in the elution of a ~30 kDa, his-tagged protein with a bright pink colour from the Ni-NTA agarose media (Figure 1).
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1030
Illegal SapI.rc site found at 827