Difference between revisions of "Part:BBa K2348011"
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<span class='h3bb'><b>Sequence and Features</b></span><br>
 
<span class='h3bb'><b>Sequence and Features</b></span><br>
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<partinfo>BBa_K2348011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2348011 SequenceAndFeatures</partinfo>
  

Revision as of 18:18, 1 November 2017


asr promoter + mCardinal
This part actually is a composite part containing the asr promoter BBa_K2348001 and mCardinal BBa_K2348002. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part BBa_K2348012

Usage and Biology

he part is used as pH regulated mCardinal expression device. Data of expression is shown in the asr Promoter part BBa_K2348001. Sequence and Features
800px-Asr_construct_Graz.jpeg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 639
    Illegal BsaI.rc site found at 828