Difference between revisions of "Part:BBa K2348011"

Revision as of 18:16, 1 November 2017

asr promoter + mCardinal
This part actually is a composite part containing the asr promoter BBa_K2348001 and mCardinal BBa_K2348002. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and f-degron allow a fast and controllable degradation of proteins and in combination with the fast maturation times of our fluorescence proteins, allow a fast colour change. This enables shorter time spans between signals when controlling the robot’s movement. Due to a change of our reactor design this feature is no longer needed because the pH is only changed in a small amount of media which is discarded after measurement. The 6xHis-tag allows detection of mNeonGreen on protein level and the T7-terminator stops translation. The part itself was created using extension PCR and overlap PCR.

Sequence and Features